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- A 2-Year Longitudinal Seroepidemiological Evaluation of Toxoplasma gondii Antibodies in a Cohort of Autochthonous Sheep from Central PortugalPublication . Almeida, Daniela; Quirino, João; Barradas, Patrícia Ferreira; da Silva, Priscilla Gomes; Pereira, Maria; Cruz, Rita; Santos, Carla; Mega, Cristina; Esteves, Fernando; Nóbrega, Carmen; Vala, Helena; Gärtner, Fátima; Amorim, Irina; Mesquita, João R.Background: Toxoplasma gondii is an important zoonosis and one of the major causes of abortion in sheep worldwide. (2) Methods: We performed a 2-year longitudinal serological antiT. gondii IgG screening on a cohort of a spatially confined population of a Portuguese autochthonous sheep breed in central Portugal. (3) Results: From the screening of the 2015 and 2016 sera, an increase of seroprevalence was observed (57.7% (95% CI: 49.9–65.3%) versus 69.1% (95% CI: 61.5–75.9), from 2015 and 2016, respectively) (p = 0.031). (4) Conclusions The present study is the first to provide prospective data on the anti-T. gondii serological status of a sheep cohort in Portugal, showing an increase in the occurrence of T. gondii. There is a need to provide a clearer understanding of T. gondii epidemiology in Portugal, ideally by implementing monitoring programs on sentinel herds, not only due to the high impact of T. gondii on animal health but also for it being a zoonosis.
- 3% polidocanol foam sclerotherapy versus hemorrhoidal artery ligation with recto anal repair in hemorrhoidal disease grades II-III: a randomized, pilot trialPublication . Neves, Sara; Falcão, Daniela; Povo, Ana; Castro-Poças, Fernando; Oliveira, Jorge; Salgueiro, PauloPurpose: polidocanol foam sclerotherapy (SP) versus doppler-guided hemorrhoidal artery ligation with recto-anal repair (HAL-RAR) in the treatment of hemorrhoidal disease (HD) was analyzed. Methods: a prospective, randomized study including patients with HD grades II and III was performed. Participants were randomly assigned (1:1) into SP or HAL-RAR, during a recruitment period between September 2019 and February 2020. Therapeutic success (Sodergren’s and bleeding scores) was the primary outcome. Other outcomes evaluated included complications and implication in the professional life. Efficacy and safety outcomes were evaluated during the eight weeks after surgery or the final SP session. Results: forty-six patients were allocated either to SP (n = 22) or HAL-RAR (n = 24). Most patients achieved therapeutic success (SP 100 % vs HAL-RAR 90.9 %, p = 0.131). Complete success was higher in the SP group (91.7 % vs 68.2 %, p = 0.045) and SP patients had less complications (25 % vs 68.2 %, p = 0.003). HAL-RAR had a greater negative impact on work activity of the patient.
- A 3-year field study to assess winter cover crops as nitrogen sources for an organic maize crop in Mediterranean PortugalPublication . Perdigão, Adelaide; Pereira, José L.S.; Moreira, Nuno; Trindade, Henrique; Coutinho, JoãoCurrent environmental concerns, the rising economic and environmental costs of mineral fertilizers and the need to respond to the limitations of N fertilization in organic farming motivate the search for alternative sources of N in maize cropping. Forage legumes used as winter cover crops may improve soil N fertility and offer benefits to the environment. The aim of this study was to examine the effects, in a factorial field experiment, of two sowing dates (SD) and nine different cover crop treatments (balansa clover, crimson clover, gland clover, arrowleaf clover, French serradella, yellow lupin, Italian ryegrass, a cover mixture and a weedy fallow) as a potential alternative N source in an organically managed maize crop. The experiment was conducted at Viseu (Portugal) for three years (2007/2008, 2008/2009 and 2011/12), in the same field. Results showed that maize grain yield was not influenced by cover crop species but was affected by SD and the higher dry matter (DM) aboveground biomass production was observed in early SD (6.1 t ha 1). The cover crops revealed their importance in maize N uptake: yellow lupin of earlier SD had the highest N uptake (83.6 kg N ha 1), but this was not significantly different from balansa clover (83.2 kg N ha 1). Ryegrass was the most efficient in depleting soil mineral N but values were just significantly lower than yellow lupin. Lupin was the least efficient, leaving more mineral N in the soil than the others cover crops, immediately before the period when the risk of leaching is high. It was concluded that, under the edaphoclimatic conditions tested, most of the tested cover crops were efficient in supplying N to maize, and balansa clover had the best performance.
- A cross-cultural study of unwillingness to consume insects in Croatia, Lithuania, Portugal, Romania, and MexicoPublication . Rosa María Mariscal-Moreno; Anayansi Escalante-Aburto; César Ozuna; Guiné, Raquel; Cristina Chuck-Hernández; Marijana Matek Sarić; Elena Bartkiene; Monica Tarcea; Lucio Rodríguez-SifuentesIntroduction Insects are a nutritious and environmentally sustainable alternative to traditional animal proteins. Because of this, their use as human food is being implemented in Europe, although this practice is not well-received in the countries of that continent. On the other hand, there are countries like Mexico, with a long tradition of insect consumption, which can serve as a model for increasing insect consumption in European countries. Methods A survey on insect consumption was conducted in Mexico, Croatia, Lithuania, Portugal, and Romania, and focused on people who had never consumed insects. The Chi-square test was performed for demographic variables; multinomial logistic regressions were used to establish relations between sociodemographic variables and respondents who had not consumed insects. Data from surveyed individuals from Mexico who have not included insects in their diet were analyzed to propose strategies to boost insect consumption in European countries. Results and discussion The percentage of respondents who had not consumed insects in Mexico was 29.7%, whereas in European countries, it ranged from 70.5 to 89.3%. The Chi-square test revealed a statistically significant difference for all analyzed sociodemographic factors. Gender, age, educational level, geographical area, and income all influenced a person to be a non-consumer of insects. We suggest using isolated insect protein or insect flour to formulate/develop food products, inform about the nutritional and health benefits of insects, integrate insects into the countries’ traditional cuisine, and reduce the costs of insects to increase their consumption in European countries.
- A cultura do Figo (Ficus carica L.): uma proposta de itinerários técnicosPublication . Gonçalves, Adriana Miranda; Costa, Cristina Amaro da; Wessel, Dulcineia FerreiraA figueira (Ficus carica L.) é cultivada em Portugal desde a Antiguidade e apresenta especificidades botânicas marcantes: o figo é uma infrutescência, designada por sicónio, que contém o verdadeiro fruto - aquénios - que se encontram fechados no seu interior, e que só podem ser polinizados por um inseto específico, o ‘Blastophaga psenes’. Este estudo caracterizou os itinerários técnicos da cultura do figo, a partir de inquéritos semiestruturados, realizados a cinco produtores, que abrangeram operações culturais, custos de instalação/manutenção, formas de financiamento e canais de escoamento. Os resultados revelam potencial de rentabilidade, dependente da gestão hídrica, da escolha varietal (tipos Comum vs. Smyrna) e do acesso a mercados de alto valor (figo fresco premium ou figo seco). O cultivo em estufa, que tem o potencial de aumentar o rendimento e estender o período de colheita, em comparação com o cultivo ao ar livre, necessita de mais estudos para identificar os fatores que podem elevar sua produtividade. As práticas agrícolas observadas são maioritariamente sustentáveis, com predomínio do controlo biológico de pragas e uso reduzido de produtos químicos. Embora os custos de instalação e manutenção sejam elevados, a valorização do produto e a diversificação de mercados indicam potencial de rentabilidade, em função das variedades. O estudo foi desenvolvido em parceira com a Inovfarmer.MED.
- Abandono escolar dos estudantes do ensino superior: o caso de um Instituto Superior Politécnico da região centroPublication . Ferreira, Manuela; Campos, Sofia; Cardoso, Ana Paula; Duarte, João; Cabral, Lídia; Margarida Correia Balula Chaves, Cláudia; Abrantes, José Luís; Guiné, Raquel
- ABC series on diagnostic parasitology part 1: the Willis methodPublication . Mesquita, João R.; Esteves, Fernando; Santos, Carla; Mega, Ana Cristina; Coelho, Catarina; Cruz, Rita; Vala, Helena; Nóbrega, CármenDiagnosis of parasitic infections in animals is an interesting task that can be developed by veterinary nurses. To perform direct identification of parasites, particularly ova present in faeces, it is possible to use faecal smears, flotation and sedimentation tests. Faecal flotation is fast and inexpensive, and can be quickly implemented as a measure for infection control. Herewith we describe the Willis method, one of the most used flotation methods.
- ABC series on diagnostic parasitology part 2: the McMaster methodPublication . Nóbrega, Cármen; Santos, Carla; Mega, Ana Cristina; Coelho, Catarina; Cruz, Rita; Vala, Helena; Esteves, Fernando; Mesquita, João R.Classical coprological methods allow for inexpensive, quick and reliable detection of parasitic elements. However, the detection of these parasitic elements may be insufficient and quantification of the parasitic burden may be required. As such, faecal egg counts can play a crucial role in providing these extra data. Herewith we describe the McMaster method, one of the most used faecal egg count methods described.
- ABC series on diagnostic parasitology part 3: the Baermann techniquePublication . Mesquita, João R.; Mega, Ana Cristina; Coelho, Catarina; Cruz, Rita; Vala, Helena; Esteves, Fernando; Santos, Carla; Nóbrega, CármenDiagnosis of parasitic larval forms is typically associated with complex, costly methods. As such, simple, low-cost diagnostic tools for larvae identification are of the utmost importance in diagnostic parasitology in the veterinary clinic. Herewith we describe the Baermann technique, one of the most used larvae identification methods
- Abstract Book of International Web Conference on Food Choice & Eating MotivationPublication . Lima, Cristina; Cunha, Ana Margarida; Pereira, Andreia; Carvalho, Renato; Dulyanska, Yuliya; Guiné, Raquel