ESCOLA SUPERIOR AGRÁRIA DE VISEU
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- A 2-Year Longitudinal Seroepidemiological Evaluation of Toxoplasma gondii Antibodies in a Cohort of Autochthonous Sheep from Central PortugalPublication . Almeida, Daniela; Quirino, João; Barradas, Patrícia Ferreira; da Silva, Priscilla Gomes; Pereira, Maria; Cruz, Rita; Santos, Carla; Mega, Cristina; Esteves, Fernando; Nóbrega, Carmen; Vala, Helena; Gärtner, Fátima; Amorim, Irina; Mesquita, João R.Background: Toxoplasma gondii is an important zoonosis and one of the major causes of abortion in sheep worldwide. (2) Methods: We performed a 2-year longitudinal serological antiT. gondii IgG screening on a cohort of a spatially confined population of a Portuguese autochthonous sheep breed in central Portugal. (3) Results: From the screening of the 2015 and 2016 sera, an increase of seroprevalence was observed (57.7% (95% CI: 49.9–65.3%) versus 69.1% (95% CI: 61.5–75.9), from 2015 and 2016, respectively) (p = 0.031). (4) Conclusions The present study is the first to provide prospective data on the anti-T. gondii serological status of a sheep cohort in Portugal, showing an increase in the occurrence of T. gondii. There is a need to provide a clearer understanding of T. gondii epidemiology in Portugal, ideally by implementing monitoring programs on sentinel herds, not only due to the high impact of T. gondii on animal health but also for it being a zoonosis.
- 3% polidocanol foam sclerotherapy versus hemorrhoidal artery ligation with recto anal repair in hemorrhoidal disease grades II-III: a randomized, pilot trialPublication . Neves, Sara; Falcão, Daniela; Povo, Ana; Castro-Poças, Fernando; Oliveira, Jorge; Salgueiro, PauloPurpose: polidocanol foam sclerotherapy (SP) versus doppler-guided hemorrhoidal artery ligation with recto-anal repair (HAL-RAR) in the treatment of hemorrhoidal disease (HD) was analyzed. Methods: a prospective, randomized study including patients with HD grades II and III was performed. Participants were randomly assigned (1:1) into SP or HAL-RAR, during a recruitment period between September 2019 and February 2020. Therapeutic success (Sodergren’s and bleeding scores) was the primary outcome. Other outcomes evaluated included complications and implication in the professional life. Efficacy and safety outcomes were evaluated during the eight weeks after surgery or the final SP session. Results: forty-six patients were allocated either to SP (n = 22) or HAL-RAR (n = 24). Most patients achieved therapeutic success (SP 100 % vs HAL-RAR 90.9 %, p = 0.131). Complete success was higher in the SP group (91.7 % vs 68.2 %, p = 0.045) and SP patients had less complications (25 % vs 68.2 %, p = 0.003). HAL-RAR had a greater negative impact on work activity of the patient.
- A 3-year field study to assess winter cover crops as nitrogen sources for an organic maize crop in Mediterranean PortugalPublication . Perdigão, Adelaide; Pereira, José L.S.; Moreira, Nuno; Trindade, Henrique; Coutinho, JoãoCurrent environmental concerns, the rising economic and environmental costs of mineral fertilizers and the need to respond to the limitations of N fertilization in organic farming motivate the search for alternative sources of N in maize cropping. Forage legumes used as winter cover crops may improve soil N fertility and offer benefits to the environment. The aim of this study was to examine the effects, in a factorial field experiment, of two sowing dates (SD) and nine different cover crop treatments (balansa clover, crimson clover, gland clover, arrowleaf clover, French serradella, yellow lupin, Italian ryegrass, a cover mixture and a weedy fallow) as a potential alternative N source in an organically managed maize crop. The experiment was conducted at Viseu (Portugal) for three years (2007/2008, 2008/2009 and 2011/12), in the same field. Results showed that maize grain yield was not influenced by cover crop species but was affected by SD and the higher dry matter (DM) aboveground biomass production was observed in early SD (6.1 t ha 1). The cover crops revealed their importance in maize N uptake: yellow lupin of earlier SD had the highest N uptake (83.6 kg N ha 1), but this was not significantly different from balansa clover (83.2 kg N ha 1). Ryegrass was the most efficient in depleting soil mineral N but values were just significantly lower than yellow lupin. Lupin was the least efficient, leaving more mineral N in the soil than the others cover crops, immediately before the period when the risk of leaching is high. It was concluded that, under the edaphoclimatic conditions tested, most of the tested cover crops were efficient in supplying N to maize, and balansa clover had the best performance.
- Abandono escolar dos estudantes do ensino superior: o caso de um Instituto Superior Politécnico da região centroPublication . Ferreira, Manuela; Campos, Sofia; Cardoso, Ana Paula; Duarte, João; Cabral, Lídia; Margarida Correia Balula Chaves, Cláudia; Abrantes, José Luís; Guiné, Raquel
- ABC series on diagnostic parasitology part 1: the Willis methodPublication . Mesquita, João R.; Esteves, Fernando; Santos, Carla; Mega, Ana Cristina; Coelho, Catarina; Cruz, Rita; Vala, Helena; Nóbrega, CármenDiagnosis of parasitic infections in animals is an interesting task that can be developed by veterinary nurses. To perform direct identification of parasites, particularly ova present in faeces, it is possible to use faecal smears, flotation and sedimentation tests. Faecal flotation is fast and inexpensive, and can be quickly implemented as a measure for infection control. Herewith we describe the Willis method, one of the most used flotation methods.
- ABC series on diagnostic parasitology part 2: the McMaster methodPublication . Nóbrega, Cármen; Santos, Carla; Mega, Ana Cristina; Coelho, Catarina; Cruz, Rita; Vala, Helena; Esteves, Fernando; Mesquita, João R.Classical coprological methods allow for inexpensive, quick and reliable detection of parasitic elements. However, the detection of these parasitic elements may be insufficient and quantification of the parasitic burden may be required. As such, faecal egg counts can play a crucial role in providing these extra data. Herewith we describe the McMaster method, one of the most used faecal egg count methods described.
- ABC series on diagnostic parasitology part 3: the Baermann techniquePublication . Mesquita, João R.; Mega, Ana Cristina; Coelho, Catarina; Cruz, Rita; Vala, Helena; Esteves, Fernando; Santos, Carla; Nóbrega, CármenDiagnosis of parasitic larval forms is typically associated with complex, costly methods. As such, simple, low-cost diagnostic tools for larvae identification are of the utmost importance in diagnostic parasitology in the veterinary clinic. Herewith we describe the Baermann technique, one of the most used larvae identification methods
- Abstract Book of International Web Conference on Food Choice & Eating MotivationPublication . Lima, Cristina; Cunha, Ana Margarida; Pereira, Andreia; Carvalho, Renato; Dulyanska, Yuliya; Guiné, Raquel
- Acheta Domesticus - Novel food ingredient: influence on cereal products quality and aclylamide contentPublication . Bartkiene, E.; Klupsaite, D.; Starkute, V.; Mockus, E.; Cernauskas, D.; Rocha, J. M.; Ozogul, F.; Guiné, RaquelIn some countries, the use of insects as food is traditional. However, in Europe, this alternative source of nutrients is still new, and consumers react differently to this offer. With the regulation of some raw materials prepared from insects in Europe, a need has arisen for their adaptation in the food industry. Today it is important to understand that consumers in Europe are not ready to choose insects as a main food source. However, the inclusion of raw materials prepared from insects into traditional food product formulas could be promising. Despite the fact that raw materials prepared from insects are considered to be of high biological value, when incorporating them into traditional food formulas, it is important to control not only the nutritional value and sensory properties of the final product, but also to anticipate what undesirable changes may occur during technological processes. One of such undesirable phenomena could be the assumption that enriching cereal products with raw materials high in protein might influence changes in the concentration of Maillard reaction products. We decided to start our research with an assessment of the acceptability of newly proposed raw materials – insects - by consumers. In the following stages, Acheta domesticus flour (unfermented and fermented with Lactiplantibacillus plantarum No. 122 and Lacticaseibacillus casei No. 210 strains) was used to enrich bread and biscuits, and their impact on product quality parameters and acrylamide concentration was assessed. Additionally, various parameters of unfermented and fermented Acheta domesticus flour were evaluated (pH, lactic acid bacteria count, color coordinates, fatty acid, volatile compound, and biogenic amine concentration). The tested product groups were prepared by adding to the main recipe different quantities of unfermented and fermented Acheta domesticus flour (into the main wheat bread formula - 10, 20, 30%; into the main biscuits formula (280 g of wheat flour, 100 g of margarine, 50 g of saccharose, 3 g of vanilla sugar, 50 g of eggs, 1.5 g of salt and 2.0 g of baking powder - 40, 80, and 100 g). An assessment of the acceptability of insects by consumers showed that 70.7% of the respondents had never eaten insects. A fermentation experiment demonstrated that in most cases, fermentation reduced the biogenic amine concentration in Acheta domesticus flour and influenced the volatile compound profile in both Acheta domesticus flour and cereal products (wheat bread and biscuits). Most of the wheat bread sample groups prepared with untreated and fermented Acheta domesticus flour showed higher acrylamide content compared to the control bread group, except for the group with 10% fermented Acheta domesticus flour. However, the opposite tendency was found for biscuit samples. The lowest acrylamide concentration (84.1 µg kg-1) was observed in biscuit samples containing 40 g of No. 210 fermented Acheta domesticus flour.Due to the decrease in acrylamide concentration in biscuits, fermented Acheta domesticus flour can be recommended for the manufacture of such products. For wheat bread preparation, 10% fermented Acheta domesticus flour is recommended, as most wheat bread sample groups prepared with untreated and fermented Acheta domesticus flour showed higher acrylamide content compared to the control bread sample groups.
- Acrilamida alimentarPublication . Martins, Evaristo de Almeida; Lemos, Edite Teixeira de; Correia, Ana CristinaNa oitava sessão do comité intergovernamental da UNESCO, decorrida no dia 4 de Dezembro de 2013 em Baku, capital do Azerbaijão e a propósito de uma candidatura plurinacional envolvendo 7 países (Portugal, Espanha, Marrocos, Itália, Grécia, Chipre e Croácia), a dieta mediterrânica (DM) foi considerada Património Imaterial da Humanidade. Um dos sinais da descaracterização da DM é o consumo excessivo de alimentos fritos. Para além da recorrência familiar a esta técnica culinária não descuremos a oferta de uma multiplicidade de produtos alimentares tipo snack (fritos) que aumentam o aporte lipídico na dieta (gorduras degradadas) e são veículo de substâncias potencialmente tóxicas como é o caso da acrilamida. As doenças cardiometabólicas (DCM ) afetam a qualidade de vida das populações. A funcionalidade do padrão mediterrânico na reabilitação da saúde mostra-nos o antídoto a estilos de vida nefastos (fast food, sedentarismo) numa sociedade de consumo. Num contexto de nutriprevenção, a slow food mediterrânica poderá ser a solução para atenuar o risco de doença e reduzir os custos em saúde. Constituiu objetivo principal do presente trabalho fundamentar a importância que a DM apresenta para a saúde cardiometabólica e avaliar os prejuízos decorrentes do consumo de alimentos submetidos a fritura, mais concretamente à batata frita (incaracterística da referida dieta). O desenvolvimento desta dissertação mostra que o balanço entre os efeitos benéficos da DM na saúde e as consequências nefastas de um produto de grande consumo (batata frita) apela à utilização esporádica do processo de fritura e à implementação de metodologias tecnológicas que diminuam o teor de acrilamida, apontando para a importância da educação/reeducação alimentar, estruturada a partir de conhecimentos ponderados da antropologia da alimentação.